Gene Therapy to Prolong Vision

Abstract: There are >200 human disease genes leading to blindness. Although gene therapy in which each disease gene is augmented or edited is possible, this approach would be extremely expensive and logistically challenging. To provide an alternative, more general approach, our laboratory has been analyzing mouse models of blindness, looking for problems that are common across genotypes. We were particularly interested in mouse models for retinitis pigmentosa (RP), as it is well modeled in mice, relative to humans. In RP, the disease starts with the expression of mutant genes in rod photoreceptors, the cell type that initiates dim light vision, leading to poor night vision. However, color vision, which originates with cone photoreceptors, is normal at birth. Over time, cones become affected due to bystander effects from rod loss. This causes color blindness and can lead to total blindness. Other cells also are affected by the loss of rods: the retinal pigmented epithelial cells (RPE), which provide various types of support to rods and cones. Studies of these mouse models led to the hypothesis that the bystander effects include: oxidative damage, metabolic shortcomings, and inflammation. To combat these problems, many different types of genes were delivered using adeno-associated viruses (AAV). Genes that fight inflammation, a transcription factor that regulates genes that fight oxidative damage, and genes that provide metabolic support were found to prolong cone and RPE survival as well as vision across 3 strains of RP mice.

biochemistrycancer biologychemical biologycell biologydevelopmental biologydrug discoveryevolutiongeneticsimmunologymolecular biologymicrobiologyneuroscienceplant sciencessystems biologybiotechnologybiological techniquesbiophysics

Audience: researchers in the discipline

Colloquium zooming Molecular & Cellular Biology LUMS

Series comments: Zooming Molecular & Cellular Biology Colloquium was initiated on July 28th, 2020 by the Biology department at Syed Babar Ali School of Science & Engineering (SBASSE), Lahore University of Management Sciences (LUMS). The series follows through various topics which come under the ambit of Molecular and Cellular Biology and we’ve had the pleasure and honor of hosting some of the most knowledgeable scientists from around the globe. Organizers can be reached out at 24100162@lums.edu.pk or 24100001@lums.edu.pk for the Zoom link of individual talks. All lectures are LIVE streamed on SBASSE's official Facebook page.